Mouse Bioarrays
CodeLink™ Mouse Whole Genome Bioarray
The Mouse Whole Genome Bioarray offers offers comprehensive genome-wide gene expression analysis on a single bioarray.
CodeLink Mouse Focused Bioarrays
CodeLink Mouse Focused Bioarrays provide the researcher with subsets of the whole genome array to enable pathway and process specific research.
The arrays are built in a 16-assay format, allowing for lower sample volume and higher assay throughput.
Applied Microarrays will partner with you to create the required subset of interest.
In addition, we offer catalog Inflammation and ADME subsets which have been compiled in collaboration with expert researchers from that field
Fig. CodeLink Mouse Whole Genome Bioarrays exhibit excellent array- to-array signal intensity reproducibility. The plotted normalized probe signals from two independent CodeLink Mouse Whole Genome Bioarrays—each run with 10 µg of the same labeled brain cRNA target—are shown here. Between these two bioarrays, 99.6% of all probes with “good” signals were within two-fold of each other. The blue data points represent the concordantly “good” probes; the red data points represent the concordantly “absent” probes. The two-fold lines are shown in gray.
Fig. CodeLink Mouse Whole Genome Bioarrays exhibit excellent reproducibility within and across bioarray production batches as represented by median % CV. These data were generated using one brain cRNA sample on three replicate arrays from one production batch (within batch, blue), and one array from each of three production batches (across batch, red).
Specifications
Mice exhibit high similarity to many human biological pathways, making it a key model system for human physiological and toxicological studies in drug discovery applications. The probe sequences, representing well annotated, full length and partial mouse gene sequences, were designed based on sequences selected from the NCBI UniGene build #139, RefSeq database (April 1, 2004 release) and dbEST database (July 31, 2004 release).Well-annotated mRNA or coding sequences were chosen to ensure usefulness for a large range of applications in basic research, biotechnology, and drug development. Each sequence was carefully screened to ensure high-quality, specific probe design, and to reduce redundancy of gene targets. All of the probes designed to these sequences were functionally tested, and over 97% were functionally validated against 14 mouse tissues to ensure best representation of the gene and biologically relevant results.
| Number of arrays in set | 1 |
| Number of probes | 35 587 (Total) 34 967 (Unique sequences) |
| Number of transcripts | 36 142 |
| Number of probes targeting transcripts other than controls |
34 967 |
| Positive controls | 300 |
| Negative controls | 320 |
| Housekeeping genes | ~50 |
| Number of probes per gene | One specific and functionally validated probe |
| Oligonucleotide probe length | 30-mer probe |
| Probe proximity to 3' end | 90% within 939 bases; average distance of 412 bases |
| Total RNA input | 0.2–2 µg total RNA |
| cRNA input | 10 µg |
| Type of assay | One color |
| Storage and handling temperature | Room temperature |